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gfp expressing escherichia coli  (ATCC)
99
ATCC gfp expressing escherichia coli
Bifidobacterium supplementation improves intestinal barrier and reduces gut-derived bacterial signals (A) Experimental flowchart. (B) Genus-level gut microbiota composition in SCI+Vehicle and SCI+Strains mice ( n = 4 per group). (C) PCoA of weighted UniFrac distances showing clustering of gut microbiota in SCI+Vehicle vs. SCI+Strains (PERMANOVA, ∗ p < 0.05; n = 4 per group). (D) Relative abundance of Bifidobacterium (genus level) (unpaired two-tailed t test; mean ± SD; ∗ p < 0.05; n = 4 per group). (E) PCoA based on MetaCyc metabolic pathway abundances showing distinct functional clustering between the SCI+Vehicle and SCI+Strains groups. (F) Functional prediction of gut microbiota metabolic pathways (MetaCyc) using PICRUSt2. Bar plot shows the top 10 differentially abundant pathways. Red bars indicate pathways enriched in the SCI+Strains group, while blue bars indicate pathways enriched in the SCI+Vehicle group (two-sided Welch’s t test, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001; n = 4 per group). (G) PGP9.5 immunohistochemistry of colon showing greater preservation of enteric neurons with supplementation (scale bars, 50 and 20 μm; n = 4 per group). (H) Immunofluorescence of ZO-1 and occludin (red; nuclei stained with 4′,6-diamidino-2-phenylindole [DAPI]) in colon (scale bars, 100 and 25 μm; n = 4 per group). <t>(I)</t> <t>GFP-expressing</t> E. coli (green) in colon (scale bars, 25 μm). (J) GFP-expressing E. coli in lung (scale bars, 25 and 10 μm; n = 4 per group). (K) BALF fluorescence (excitation 488 nm, emission 510–530 nm) showing reduced signal in SCI+Strains (mean ± SD; ∗∗ p < 0.01; unpaired two-tailed t test; n = 4 per group). (L) Representative H&E-stained lung sections (scale bars, 100 μm).
Gfp Expressing Escherichia Coli, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gfp expression  (Sartorius AG)
99
Sartorius AG gfp expression
Bifidobacterium supplementation improves intestinal barrier and reduces gut-derived bacterial signals (A) Experimental flowchart. (B) Genus-level gut microbiota composition in SCI+Vehicle and SCI+Strains mice ( n = 4 per group). (C) PCoA of weighted UniFrac distances showing clustering of gut microbiota in SCI+Vehicle vs. SCI+Strains (PERMANOVA, ∗ p < 0.05; n = 4 per group). (D) Relative abundance of Bifidobacterium (genus level) (unpaired two-tailed t test; mean ± SD; ∗ p < 0.05; n = 4 per group). (E) PCoA based on MetaCyc metabolic pathway abundances showing distinct functional clustering between the SCI+Vehicle and SCI+Strains groups. (F) Functional prediction of gut microbiota metabolic pathways (MetaCyc) using PICRUSt2. Bar plot shows the top 10 differentially abundant pathways. Red bars indicate pathways enriched in the SCI+Strains group, while blue bars indicate pathways enriched in the SCI+Vehicle group (two-sided Welch’s t test, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001; n = 4 per group). (G) PGP9.5 immunohistochemistry of colon showing greater preservation of enteric neurons with supplementation (scale bars, 50 and 20 μm; n = 4 per group). (H) Immunofluorescence of ZO-1 and occludin (red; nuclei stained with 4′,6-diamidino-2-phenylindole [DAPI]) in colon (scale bars, 100 and 25 μm; n = 4 per group). <t>(I)</t> <t>GFP-expressing</t> E. coli (green) in colon (scale bars, 25 μm). (J) GFP-expressing E. coli in lung (scale bars, 25 and 10 μm; n = 4 per group). (K) BALF fluorescence (excitation 488 nm, emission 510–530 nm) showing reduced signal in SCI+Strains (mean ± SD; ∗∗ p < 0.01; unpaired two-tailed t test; n = 4 per group). (L) Representative H&E-stained lung sections (scale bars, 100 μm).
Gfp Expression, supplied by Sartorius AG, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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expression plasmid  (Proteintech)
96
Proteintech expression plasmid
Bifidobacterium supplementation improves intestinal barrier and reduces gut-derived bacterial signals (A) Experimental flowchart. (B) Genus-level gut microbiota composition in SCI+Vehicle and SCI+Strains mice ( n = 4 per group). (C) PCoA of weighted UniFrac distances showing clustering of gut microbiota in SCI+Vehicle vs. SCI+Strains (PERMANOVA, ∗ p < 0.05; n = 4 per group). (D) Relative abundance of Bifidobacterium (genus level) (unpaired two-tailed t test; mean ± SD; ∗ p < 0.05; n = 4 per group). (E) PCoA based on MetaCyc metabolic pathway abundances showing distinct functional clustering between the SCI+Vehicle and SCI+Strains groups. (F) Functional prediction of gut microbiota metabolic pathways (MetaCyc) using PICRUSt2. Bar plot shows the top 10 differentially abundant pathways. Red bars indicate pathways enriched in the SCI+Strains group, while blue bars indicate pathways enriched in the SCI+Vehicle group (two-sided Welch’s t test, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001; n = 4 per group). (G) PGP9.5 immunohistochemistry of colon showing greater preservation of enteric neurons with supplementation (scale bars, 50 and 20 μm; n = 4 per group). (H) Immunofluorescence of ZO-1 and occludin (red; nuclei stained with 4′,6-diamidino-2-phenylindole [DAPI]) in colon (scale bars, 100 and 25 μm; n = 4 per group). <t>(I)</t> <t>GFP-expressing</t> E. coli (green) in colon (scale bars, 25 μm). (J) GFP-expressing E. coli in lung (scale bars, 25 and 10 μm; n = 4 per group). (K) BALF fluorescence (excitation 488 nm, emission 510–530 nm) showing reduced signal in SCI+Strains (mean ± SD; ∗∗ p < 0.01; unpaired two-tailed t test; n = 4 per group). (L) Representative H&E-stained lung sections (scale bars, 100 μm).
Expression Plasmid, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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expression plasmid - by Bioz Stars, 2026-02
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gfp luciferase expressing sublines  (ATCC)
99
ATCC gfp luciferase expressing sublines
Bifidobacterium supplementation improves intestinal barrier and reduces gut-derived bacterial signals (A) Experimental flowchart. (B) Genus-level gut microbiota composition in SCI+Vehicle and SCI+Strains mice ( n = 4 per group). (C) PCoA of weighted UniFrac distances showing clustering of gut microbiota in SCI+Vehicle vs. SCI+Strains (PERMANOVA, ∗ p < 0.05; n = 4 per group). (D) Relative abundance of Bifidobacterium (genus level) (unpaired two-tailed t test; mean ± SD; ∗ p < 0.05; n = 4 per group). (E) PCoA based on MetaCyc metabolic pathway abundances showing distinct functional clustering between the SCI+Vehicle and SCI+Strains groups. (F) Functional prediction of gut microbiota metabolic pathways (MetaCyc) using PICRUSt2. Bar plot shows the top 10 differentially abundant pathways. Red bars indicate pathways enriched in the SCI+Strains group, while blue bars indicate pathways enriched in the SCI+Vehicle group (two-sided Welch’s t test, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001; n = 4 per group). (G) PGP9.5 immunohistochemistry of colon showing greater preservation of enteric neurons with supplementation (scale bars, 50 and 20 μm; n = 4 per group). (H) Immunofluorescence of ZO-1 and occludin (red; nuclei stained with 4′,6-diamidino-2-phenylindole [DAPI]) in colon (scale bars, 100 and 25 μm; n = 4 per group). <t>(I)</t> <t>GFP-expressing</t> E. coli (green) in colon (scale bars, 25 μm). (J) GFP-expressing E. coli in lung (scale bars, 25 and 10 μm; n = 4 per group). (K) BALF fluorescence (excitation 488 nm, emission 510–530 nm) showing reduced signal in SCI+Strains (mean ± SD; ∗∗ p < 0.01; unpaired two-tailed t test; n = 4 per group). (L) Representative H&E-stained lung sections (scale bars, 100 μm).
Gfp Luciferase Expressing Sublines, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gfp spark trdmt1  (Sino Biological)
90
Sino Biological gfp spark trdmt1
Bifidobacterium supplementation improves intestinal barrier and reduces gut-derived bacterial signals (A) Experimental flowchart. (B) Genus-level gut microbiota composition in SCI+Vehicle and SCI+Strains mice ( n = 4 per group). (C) PCoA of weighted UniFrac distances showing clustering of gut microbiota in SCI+Vehicle vs. SCI+Strains (PERMANOVA, ∗ p < 0.05; n = 4 per group). (D) Relative abundance of Bifidobacterium (genus level) (unpaired two-tailed t test; mean ± SD; ∗ p < 0.05; n = 4 per group). (E) PCoA based on MetaCyc metabolic pathway abundances showing distinct functional clustering between the SCI+Vehicle and SCI+Strains groups. (F) Functional prediction of gut microbiota metabolic pathways (MetaCyc) using PICRUSt2. Bar plot shows the top 10 differentially abundant pathways. Red bars indicate pathways enriched in the SCI+Strains group, while blue bars indicate pathways enriched in the SCI+Vehicle group (two-sided Welch’s t test, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001; n = 4 per group). (G) PGP9.5 immunohistochemistry of colon showing greater preservation of enteric neurons with supplementation (scale bars, 50 and 20 μm; n = 4 per group). (H) Immunofluorescence of ZO-1 and occludin (red; nuclei stained with 4′,6-diamidino-2-phenylindole [DAPI]) in colon (scale bars, 100 and 25 μm; n = 4 per group). <t>(I)</t> <t>GFP-expressing</t> E. coli (green) in colon (scale bars, 25 μm). (J) GFP-expressing E. coli in lung (scale bars, 25 and 10 μm; n = 4 per group). (K) BALF fluorescence (excitation 488 nm, emission 510–530 nm) showing reduced signal in SCI+Strains (mean ± SD; ∗∗ p < 0.01; unpaired two-tailed t test; n = 4 per group). (L) Representative H&E-stained lung sections (scale bars, 100 μm).
Gfp Spark Trdmt1, supplied by Sino Biological, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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zc3h12d gfp fusion protein  (Proteintech)
93
Proteintech zc3h12d gfp fusion protein
Bifidobacterium supplementation improves intestinal barrier and reduces gut-derived bacterial signals (A) Experimental flowchart. (B) Genus-level gut microbiota composition in SCI+Vehicle and SCI+Strains mice ( n = 4 per group). (C) PCoA of weighted UniFrac distances showing clustering of gut microbiota in SCI+Vehicle vs. SCI+Strains (PERMANOVA, ∗ p < 0.05; n = 4 per group). (D) Relative abundance of Bifidobacterium (genus level) (unpaired two-tailed t test; mean ± SD; ∗ p < 0.05; n = 4 per group). (E) PCoA based on MetaCyc metabolic pathway abundances showing distinct functional clustering between the SCI+Vehicle and SCI+Strains groups. (F) Functional prediction of gut microbiota metabolic pathways (MetaCyc) using PICRUSt2. Bar plot shows the top 10 differentially abundant pathways. Red bars indicate pathways enriched in the SCI+Strains group, while blue bars indicate pathways enriched in the SCI+Vehicle group (two-sided Welch’s t test, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001; n = 4 per group). (G) PGP9.5 immunohistochemistry of colon showing greater preservation of enteric neurons with supplementation (scale bars, 50 and 20 μm; n = 4 per group). (H) Immunofluorescence of ZO-1 and occludin (red; nuclei stained with 4′,6-diamidino-2-phenylindole [DAPI]) in colon (scale bars, 100 and 25 μm; n = 4 per group). <t>(I)</t> <t>GFP-expressing</t> E. coli (green) in colon (scale bars, 25 μm). (J) GFP-expressing E. coli in lung (scale bars, 25 and 10 μm; n = 4 per group). (K) BALF fluorescence (excitation 488 nm, emission 510–530 nm) showing reduced signal in SCI+Strains (mean ± SD; ∗∗ p < 0.01; unpaired two-tailed t test; n = 4 per group). (L) Representative H&E-stained lung sections (scale bars, 100 μm).
Zc3h12d Gfp Fusion Protein, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nudt5 gfp  (Proteintech)
93
Proteintech nudt5 gfp
Bifidobacterium supplementation improves intestinal barrier and reduces gut-derived bacterial signals (A) Experimental flowchart. (B) Genus-level gut microbiota composition in SCI+Vehicle and SCI+Strains mice ( n = 4 per group). (C) PCoA of weighted UniFrac distances showing clustering of gut microbiota in SCI+Vehicle vs. SCI+Strains (PERMANOVA, ∗ p < 0.05; n = 4 per group). (D) Relative abundance of Bifidobacterium (genus level) (unpaired two-tailed t test; mean ± SD; ∗ p < 0.05; n = 4 per group). (E) PCoA based on MetaCyc metabolic pathway abundances showing distinct functional clustering between the SCI+Vehicle and SCI+Strains groups. (F) Functional prediction of gut microbiota metabolic pathways (MetaCyc) using PICRUSt2. Bar plot shows the top 10 differentially abundant pathways. Red bars indicate pathways enriched in the SCI+Strains group, while blue bars indicate pathways enriched in the SCI+Vehicle group (two-sided Welch’s t test, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001; n = 4 per group). (G) PGP9.5 immunohistochemistry of colon showing greater preservation of enteric neurons with supplementation (scale bars, 50 and 20 μm; n = 4 per group). (H) Immunofluorescence of ZO-1 and occludin (red; nuclei stained with 4′,6-diamidino-2-phenylindole [DAPI]) in colon (scale bars, 100 and 25 μm; n = 4 per group). <t>(I)</t> <t>GFP-expressing</t> E. coli (green) in colon (scale bars, 25 μm). (J) GFP-expressing E. coli in lung (scale bars, 25 and 10 μm; n = 4 per group). (K) BALF fluorescence (excitation 488 nm, emission 510–530 nm) showing reduced signal in SCI+Strains (mean ± SD; ∗∗ p < 0.01; unpaired two-tailed t test; n = 4 per group). (L) Representative H&E-stained lung sections (scale bars, 100 μm).
Nudt5 Gfp, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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asc gfp  (Sino Biological)
94
Sino Biological asc gfp
Bifidobacterium supplementation improves intestinal barrier and reduces gut-derived bacterial signals (A) Experimental flowchart. (B) Genus-level gut microbiota composition in SCI+Vehicle and SCI+Strains mice ( n = 4 per group). (C) PCoA of weighted UniFrac distances showing clustering of gut microbiota in SCI+Vehicle vs. SCI+Strains (PERMANOVA, ∗ p < 0.05; n = 4 per group). (D) Relative abundance of Bifidobacterium (genus level) (unpaired two-tailed t test; mean ± SD; ∗ p < 0.05; n = 4 per group). (E) PCoA based on MetaCyc metabolic pathway abundances showing distinct functional clustering between the SCI+Vehicle and SCI+Strains groups. (F) Functional prediction of gut microbiota metabolic pathways (MetaCyc) using PICRUSt2. Bar plot shows the top 10 differentially abundant pathways. Red bars indicate pathways enriched in the SCI+Strains group, while blue bars indicate pathways enriched in the SCI+Vehicle group (two-sided Welch’s t test, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001; n = 4 per group). (G) PGP9.5 immunohistochemistry of colon showing greater preservation of enteric neurons with supplementation (scale bars, 50 and 20 μm; n = 4 per group). (H) Immunofluorescence of ZO-1 and occludin (red; nuclei stained with 4′,6-diamidino-2-phenylindole [DAPI]) in colon (scale bars, 100 and 25 μm; n = 4 per group). <t>(I)</t> <t>GFP-expressing</t> E. coli (green) in colon (scale bars, 25 μm). (J) GFP-expressing E. coli in lung (scale bars, 25 and 10 μm; n = 4 per group). (K) BALF fluorescence (excitation 488 nm, emission 510–530 nm) showing reduced signal in SCI+Strains (mean ± SD; ∗∗ p < 0.01; unpaired two-tailed t test; n = 4 per group). (L) Representative H&E-stained lung sections (scale bars, 100 μm).
Asc Gfp, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/asc gfp/product/Sino Biological
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Image Search Results


Bifidobacterium supplementation improves intestinal barrier and reduces gut-derived bacterial signals (A) Experimental flowchart. (B) Genus-level gut microbiota composition in SCI+Vehicle and SCI+Strains mice ( n = 4 per group). (C) PCoA of weighted UniFrac distances showing clustering of gut microbiota in SCI+Vehicle vs. SCI+Strains (PERMANOVA, ∗ p < 0.05; n = 4 per group). (D) Relative abundance of Bifidobacterium (genus level) (unpaired two-tailed t test; mean ± SD; ∗ p < 0.05; n = 4 per group). (E) PCoA based on MetaCyc metabolic pathway abundances showing distinct functional clustering between the SCI+Vehicle and SCI+Strains groups. (F) Functional prediction of gut microbiota metabolic pathways (MetaCyc) using PICRUSt2. Bar plot shows the top 10 differentially abundant pathways. Red bars indicate pathways enriched in the SCI+Strains group, while blue bars indicate pathways enriched in the SCI+Vehicle group (two-sided Welch’s t test, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001; n = 4 per group). (G) PGP9.5 immunohistochemistry of colon showing greater preservation of enteric neurons with supplementation (scale bars, 50 and 20 μm; n = 4 per group). (H) Immunofluorescence of ZO-1 and occludin (red; nuclei stained with 4′,6-diamidino-2-phenylindole [DAPI]) in colon (scale bars, 100 and 25 μm; n = 4 per group). (I) GFP-expressing E. coli (green) in colon (scale bars, 25 μm). (J) GFP-expressing E. coli in lung (scale bars, 25 and 10 μm; n = 4 per group). (K) BALF fluorescence (excitation 488 nm, emission 510–530 nm) showing reduced signal in SCI+Strains (mean ± SD; ∗∗ p < 0.01; unpaired two-tailed t test; n = 4 per group). (L) Representative H&E-stained lung sections (scale bars, 100 μm).

Journal: iScience

Article Title: Gut-lung axis perturbation and Bifidobacterium potential after spinal cord injury in humans and mice

doi: 10.1016/j.isci.2026.114655

Figure Lengend Snippet: Bifidobacterium supplementation improves intestinal barrier and reduces gut-derived bacterial signals (A) Experimental flowchart. (B) Genus-level gut microbiota composition in SCI+Vehicle and SCI+Strains mice ( n = 4 per group). (C) PCoA of weighted UniFrac distances showing clustering of gut microbiota in SCI+Vehicle vs. SCI+Strains (PERMANOVA, ∗ p < 0.05; n = 4 per group). (D) Relative abundance of Bifidobacterium (genus level) (unpaired two-tailed t test; mean ± SD; ∗ p < 0.05; n = 4 per group). (E) PCoA based on MetaCyc metabolic pathway abundances showing distinct functional clustering between the SCI+Vehicle and SCI+Strains groups. (F) Functional prediction of gut microbiota metabolic pathways (MetaCyc) using PICRUSt2. Bar plot shows the top 10 differentially abundant pathways. Red bars indicate pathways enriched in the SCI+Strains group, while blue bars indicate pathways enriched in the SCI+Vehicle group (two-sided Welch’s t test, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001; n = 4 per group). (G) PGP9.5 immunohistochemistry of colon showing greater preservation of enteric neurons with supplementation (scale bars, 50 and 20 μm; n = 4 per group). (H) Immunofluorescence of ZO-1 and occludin (red; nuclei stained with 4′,6-diamidino-2-phenylindole [DAPI]) in colon (scale bars, 100 and 25 μm; n = 4 per group). (I) GFP-expressing E. coli (green) in colon (scale bars, 25 μm). (J) GFP-expressing E. coli in lung (scale bars, 25 and 10 μm; n = 4 per group). (K) BALF fluorescence (excitation 488 nm, emission 510–530 nm) showing reduced signal in SCI+Strains (mean ± SD; ∗∗ p < 0.01; unpaired two-tailed t test; n = 4 per group). (L) Representative H&E-stained lung sections (scale bars, 100 μm).

Article Snippet: GFP-expressing Escherichia coli , ATCC , Cat# 25922.

Techniques: Derivative Assay, Two Tailed Test, Functional Assay, Immunohistochemistry, Preserving, Immunofluorescence, Staining, Expressing, Fluorescence